rabbit polyclonal antibody against ns5b (Covance)
90
Structured Review
Covance
rabbit polyclonal antibody against ns5b
Rabbit Polyclonal Antibody Against Ns5b, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+ns5b/rabbit+polyclonal+phospho+specific+anti+thr+p++2332+ns5a/pmc05766933-331-0-8
Average 90 stars, based on 1 article reviews
Rabbit Polyclonal Antibody Against Ns5b, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+ns5b/rabbit+polyclonal+phospho+specific+anti+thr+p++2332+ns5a/pmc05766933-331-0-8
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against ns5b - by Bioz Stars,
2026-10
90/100 stars
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Recombinant:Article Title: Interferon regulatory factor 5 (IRF5) suppresses hepatitis C virus (HCV) replication and HCV-associated hepatocellular carcinoma Article Snippet: Antibodies to BCL2L1, XIAP, FADD, p300, CREB, DAB2IP, VEGF, Met, NFκBp65, Arc1, PDGFR, TRAF1, Nme2, and 14-3-3τ were from Fisher, and IRF5 and Cox2 antibodies were from Abcam (Cambridge, MA). .. Article Title: Characterization of Aurintricarboxylic Acid as a Potent Hepatitis C Virus Replicase Inhibitor Article Snippet: Compound ATA was obtained from Sigma-Aldrich (Saint Louis, MO, USA). .. 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Article Title: Characterization of Aurintricarboxylic Acid as a Potent Hepatitis C Virus Replicase Inhibitor Article Snippet: Compound ATA was obtained from Sigma-Aldrich (Saint Louis, MO, USA). .. Reagents, antibodies and cell culture medium The source of materials used in this work were as follows: nickel-nitrilotriacetic acid agarose (Ni-NTA) and NAP-10 columns from GE Healthcare (Piscataway, NJ, USA); radiolabelled [α-32P] rNTPs from Perkin Elmer (Waltham, MA, USA); [14C]-foscarnet from Moravek Biochemicals (Brea, CA, USA); HPLC-grade nucleoside triphosphates, RNase Out, glycogen and CompleteTM protease inhibitor cocktail from Roche (Mannheim, Germany); GF-B filters from Whatman (Piscataway, NJ, USA); T4 polynucleotide kinase from Invitrogen (Carlsbad, CA, USA); western blotting luminol reagent from Santa Cruz Biotechnology (Santa Cruz, CA, USA); MEGAscript T7 transcription kit from Ambion (Austin, TX, USA); RNeasy kit from Qiagen (Valencia, CA, USA); Cell Culture:Article Title: Characterization of Aurintricarboxylic Acid as a Potent Hepatitis C Virus Replicase Inhibitor Article Snippet: Compound ATA was obtained from Sigma-Aldrich (Saint Louis, MO, USA). .. 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![FIG. 8. HCV-infected cells do not accumulate in M phase when treated with microtubule inhibitors that activate the mitotic spindle checkpoint. (A) Schematic diagram showing experimental design (left) and immunoblot analysis (right) of phospho-histone H3(Ser10) abundance in Huh7.5 cell lysates that were mock infected (M) or infected with HCV HJ3-5 at an MOI of 2 for 3, 4, or 5 days. Blots for core and NS5B expression are shown to demonstrate HCV infection, and -tubulin is shown as a loading control. (B) Bright-field microscopy images of Huh7.5 cells that were either mock infected or infected with HCV(JFH1) (MOI, 2) for 3 days prior to treatment with nocodazole (Noc; 266 nM), paclitaxel (Pac; 1 M), or dimethyl sulfoxide (DMSO [0.1%, vol/vol]) for 24 h. (C) Immunoblot analysis of markers of mitosis in lysates of mock- or HCV-infected Huh7.5 cells. Cell lysates were prepared from the cells shown in panel B and subjected to SDS-PAGE and immunoblot analysis using specific antibodies to detect the indicated proteins. (D) Flow cytometry analysis of phosphorylated histone H3(Ser10) abundance. Cells were electroporated with the full-length HCV genomic RNA of JFH1 or H77Sv3 or their cognate nonreplicating mutants, JFH1/GND or H77S/AAG, cultured for 4 days, and treated with 266 nM nocodazole for different times before harvest. Cells were stained for HCV core protein and phospho-histone H3. The percentages of cells positive for phospho-histone H3 are shown at different time points following nocodazole treatment.](https://doi-unpaywalled-images-cdn.bioz.com/0759/10__1128_slash_jvi__00280___11/10__1128_slash_jvi__00280___11____page10_image1.jpg)